3404 genomes  (ATCC)

Journal: Mycoses

Article Title: Integrated Genomic and Phenotypic Analyses Reveal Convergent Resistance Patterns in Clinical Candida tropicalis Isolates

doi: 10.1111/myc.70181

Figure Lengend Snippet: SNP‐based phylogenetic tree of hospital isolates and the ATCC control reference genome, C. tropicalis MYA‐3404.

Article Snippet: SNPs of the ATCC C. tropicalis MYA‐3404 genomes were compared to the SNPs of the isolates by using the snippy multicommand (snippy‐base application v4.5.0) [ ] that generates a core genome multiple alignment against a common reference.

Techniques: Control

Journal: Mycoses

Article Title: Integrated Genomic and Phenotypic Analyses Reveal Convergent Resistance Patterns in Clinical Candida tropicalis Isolates

doi: 10.1111/myc.70181

Figure Lengend Snippet: Quantification of ergosterol content. The black bar represents the control strain C. tropicalis MYA‐3404, that is sensitive to antifungal agents. The grey bars represent the hospital isolates. Statistical significance was calculated using One‐way ANOVA ( p < 0.0001) coupled to Dunnett's multiple comparisons test: Control versus 46: p = 0.4182; Control versus 47: p = 0.0009; Control versus 50: p < 0.0001; Control versus 52: p < 0.0001; Control versus 53: p < 0.0001; Control versus 54: p = 0.9957; Control versus 57: p = 0.0066; Control versus 58: p = 0.0838; Control versus 60: p = 0.0051; Control versus 61: p = 0.9997; Control versus 62: p = 0.0004; Control versus 63: p = 0.0003; Control versus 65: p = 0.0032; Control versus 66: p = 0.2012.

Article Snippet: SNPs of the ATCC C. tropicalis MYA‐3404 genomes were compared to the SNPs of the isolates by using the snippy multicommand (snippy‐base application v4.5.0) [ ] that generates a core genome multiple alignment against a common reference.

Techniques: Control

Journal: Mycoses

Article Title: Integrated Genomic and Phenotypic Analyses Reveal Convergent Resistance Patterns in Clinical Candida tropicalis Isolates

doi: 10.1111/myc.70181

Figure Lengend Snippet: Adhesion to human cells. The black bar represents the control strain C. tropicalis MYA‐3404, that is sensitive to antifungal agents. The grey bars represent the hospital isolates. Statistical significance was calculated using One‐way ANOVA ( p < 0.0001) coupled to Dunnett's multiple comparisons test: Control versus 46: p = 0.0001; Control versus 47: p = 0.0283; Control versus 50: p = 0.9997; Control versus 52: p = 0.9341; Control versus 53: p = 0.002; Control versus 54: p = 0.9999; Control versus 57: p > 0.9999; Control versus 58: p = 0.1689; Control versus 60: p = 0.0016; Control versus 61: p = 0.8017; Control versus 62: p = 0.0027; Control versus 63: p = 0.0035; Control versus 65: p = 0.9653; Control versus 66: p = 0.6953.

Article Snippet: SNPs of the ATCC C. tropicalis MYA‐3404 genomes were compared to the SNPs of the isolates by using the snippy multicommand (snippy‐base application v4.5.0) [ ] that generates a core genome multiple alignment against a common reference.

Techniques: Control

Journal: Mycoses

Article Title: Integrated Genomic and Phenotypic Analyses Reveal Convergent Resistance Patterns in Clinical Candida tropicalis Isolates

doi: 10.1111/myc.70181

Figure Lengend Snippet: Biofilm formation. The black bar represents the control strain C. tropicalis MYA‐3404, that is sensitive to antifungal agents. The grey bars represent the hospital isolates. Statistical significance was calculated using one‐way ANOVA ( p < 0.0001) coupled to Dunnett's multiple comparisons test: Control versus 46: p = 0.0001; Control versus 47: p < 0.0001; Control versus 50: p < 0.0001; Control versus 52: p = 0.0588; Control versus 53: p = 0.0221; Control versus 54: p < 0.0001; Control versus 57: p = 0.9993; Control versus 58: p = 0.0644; Control versus 60: p = 0.5937; Control versus 61: p < 0.0001; Control versus 62: p = 0.1254; Control versus 63: p = 0.0971; Control versus 65: p = 0.5359; Control versus 66: p < 0.0001.

Article Snippet: SNPs of the ATCC C. tropicalis MYA‐3404 genomes were compared to the SNPs of the isolates by using the snippy multicommand (snippy‐base application v4.5.0) [ ] that generates a core genome multiple alignment against a common reference.

Techniques: Control

Journal: Mycoses

Article Title: Integrated Genomic and Phenotypic Analyses Reveal Convergent Resistance Patterns in Clinical Candida tropicalis Isolates

doi: 10.1111/myc.70181

Figure Lengend Snippet: Quantification of chitin content. The black bar represents the control strain C. tropicalis MYA‐3404, that is sensitive to antifungal agents. The grey bars represent the hospital isolates. Statistical significance was calculated using One‐way ANOVA ( p < 0.0001) coupled to Dunnett's multiple comparisons test: Control versus 46: p = 0.1409; Control versus 47: p = 0.9911; Control versus 50: p = 0.9996; Control versus 52: p = 0.0012; Control versus 53: p = 0.408; Control versus 54: p = 0.408; Control versus 57: p = 0.0034; Control versus 58: p = 0.0024; Control versus 60: p < 0.0001; Control versus 61: p = 0.0007; Control versus 62: p = 0.0005; Control versus 63: p < 0.0001; Control versus 65: p = 0.0403; Control versus 66: p < 0.0001.

Article Snippet: SNPs of the ATCC C. tropicalis MYA‐3404 genomes were compared to the SNPs of the isolates by using the snippy multicommand (snippy‐base application v4.5.0) [ ] that generates a core genome multiple alignment against a common reference.

Techniques: Control

Journal: PLoS ONE

Article Title: Indole-3-acetic acid synthesized through the indole-3-pyruvate pathway promotes Candida tropicalis biofilm formation

doi: 10.1371/journal.pone.0244246

Figure Lengend Snippet: a) C . tropicalis (1 x 10 7 cells/mL each) grown on PDA plates for 24 h in the presence or absence of IAA and colony forming units (CFUs) were counted. The results are the means from triplicate experiments and error bars indicate the means ± standard deviations. * denotes statistical significance, p-value < 0.05. b) Representative SEM images of C . tropicalis grown in the presence or absence of IAA, confirming that the addition of IAA promoted the formation of biofilms.

Article Snippet: The presence of genes coding the three enzymes involved in the indole-3-pyruvate pathway in the C . tropicalis genome (ATCC MYA-3404) also supports our conclusion.

Techniques:

Journal: PLoS ONE

Article Title: Indole-3-acetic acid synthesized through the indole-3-pyruvate pathway promotes Candida tropicalis biofilm formation

doi: 10.1371/journal.pone.0244246

Figure Lengend Snippet: C . tropicalis (0.3 x 107 cells/mL each) was grown in PBS for 0, 1, or 3 h in the presence or absence of IAA. At the indicated time points, an aliquot was taken and the numbers of total cells and germinated cells were counted. The results are the means from triplicate experiments and error bars indicate the means ± standard deviations. * denotes statistical significance, p-value < 0.05.

Article Snippet: The presence of genes coding the three enzymes involved in the indole-3-pyruvate pathway in the C . tropicalis genome (ATCC MYA-3404) also supports our conclusion.

Techniques:

Journal: PLoS ONE

Article Title: Indole-3-acetic acid synthesized through the indole-3-pyruvate pathway promotes Candida tropicalis biofilm formation

doi: 10.1371/journal.pone.0244246

Figure Lengend Snippet: C . tropicalis was initially cultured in a regular RPMI 1640 for 8 h (0 h) and the culture medium was changed to an 13 C 11 -Trp-RPMI 1640 medium and the culture was continued another 16 or 24 h. The results are the means from triplicate experiments and error bars indicate the means ± standard deviations.

Article Snippet: The presence of genes coding the three enzymes involved in the indole-3-pyruvate pathway in the C . tropicalis genome (ATCC MYA-3404) also supports our conclusion.

Techniques: Cell Culture